Annotated Non-cardiomyocytes from Integrated scRNA-seq Heart Dataset

This is a subset of a dataset created from the following public datasets: Tucker et al. (2020) DOI: 10.1161/CIRCULATIONAHA.119.045401 Livinukova (2020) DOI: 10.1038/s41586-020-2797-4 Kanemaru et al. et al. (2023) DOI: 10.1038/s41586-023-06311-1 Yang et al. (2023) DOI: 10.1002/ctm2.1297 Wang et al. (2020) DOI: 10.1038/s41556-019-0446-7 Hulsmans et al. (2023) DOI: 10.1126/science.abq3061 The combined datasets were filtered based on QC metrics, integrated using Harmony, and clustered with Scanpy. After cardiomyocyte clusters were identified and removed, the remaining cells were re-clustered and annotated based on the expression of canonical markers. An unnormalised, annotated version of the dataset is shared here as an AnnData file, together with a text document describing the original datasets and metadata fields.