The transcription factor HHEX maintains glucocorticoid levels and protects adrenals from androgen-induced lipid depletion

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To achieve single-cell resolution of the adrenocortical transcriptome, we selectively labeled steroidogenic adrenocortical cells using a transgenic Cre-LoxP approach. We combined Sf1-Cre mice with Rosa mT/mG animals to obtain mice expressing fluorescent reporter proteins at the cell membrane (mT/mG) of cells that express or have expressed the transcription factor SF-1. ). Libraries for scRNA-seq were generated using the 10X Genomics platform.