Altered glucose metabolism in proximal tubule cells responding to acute kidney injury is associated with patient mortality. Altered glucose metabolism in proximal tubule cells responding to acute kidney injury is associated with patient mortality

Single-cell transcriptomics revealed that gluconeogenesis is impaired in proximal tubule cells during AKI in mice. Overall design: To investigate single cell transcriptomics on renal tubule cells, we generated a reporter mouse expressing GFP linked to the histone protein H2B in all nephron cells by combining 3 transgenes Six2-TGC; Rosa26rtTA ; pTRE-H2BeGFP. IRI and Sham surgeries were performed, and kidney tissue was harvested at different timepoints, followed by nuclei isolation, FACs sorting, and 10X Genomics Chromium Drop-Seq and library construction. Illumina Next-Seq sequencing of barcoded single nuclei libraries was performed and aligned to a pre-mrna genome with STAR/CellRanger. Gene/barcode matrices were merged and processed with the Seurat R package.