Orsay virus infection increases Caenorhabditis elegans resistance to heat-shock

The heat shock response plays a role in the immune defense against viruses across various organisms. Studies on model organisms show that inducing this response prior to viral exposure enhances host resistance to infections, while deficient responses make individuals more vulnerable. Moreover, viruses rely on components of the heat shock response for their own stability and viral infections improve thermal tolerance in plants, giving infected individuals an advantage in extreme conditions, which aids the virus in replication and transmission. Here, we examine the interaction between the nematode Caenorhabditis elegans and its natural pathogen the Orsay virus (OrV) under heat stress. We found that OrV infection leads to differential expression of heat-stress related genes, and infected populations showed increased resistance to heat shock. This resistance correlated with increased expression of argonautes alg-1 and alg-2, which are crucial for survival after heat shock and for OrV replic..., (a) Overlap of heat-stress related GO terms and OrV DEGs
The differentially expressed genes upon OrV infection in wild-type animals were obtained from. The genes belonging to the GO term categories (i) cellular response to unfolded proteins, (ii) heat-shock binding proteins and (iii) response to heat were extracted from WormBase Ontology Browser. Only genes annotated as directly involved in each category were used. Genes that were differentially expressed upon OrV infection and belonged to any of these three GO term categories were selected.
(b) Strain maintenance
Nematodes were maintained at 20 ºC on Nematode Growth Medium (NGM) plates seeded with Escherichia coli OP50 under standard conditions. ERT54 (jyIs8 [pals-5p::GFP; myo-2p::mCherry]X in an N2 Bristol background) served as wild-type. JU2624, (mjIs228[myo-2p::mCherry::unc54; lys-3p::eGFP::tbb-2] in a JU1580 background) was the natural isolate from which OrV was first identified. Both strains were generously provided by Prof. M.A. ..., , # Orsay virus infection increases *Caenorhabditis elegans* resistance to heat-shock

[https://doi.org/10.5061/dryad.866t1g1zs](https://doi.org/10.5061/dryad.866t1g1zs)

Excel files containing survival of infected and noninfected animals in control and heat-shock conditions.  Each file corresponds to a different experimental block.  Data necessary to reproduce Figure 1c.

Excel file containing the expression of *alg-1* and *alg-2* argonaute genes. Data necessary to reproduce Figure 1d.

## Description of the data and file structure

Excel file 1: Heat shock 210902.xlsx
*C. elegans* strains JU2624 and ERT75 in control (+ M9 salts) and inoculated (+ OrV) treatments. Number of alive and dead animals. Probability of survival.

Excel file 2: Heat shock 210820.xlsx
*C. elegans* strains JU2624 and ERT75 in control (+ M9 salts) and inoculated (+ OrV) treatments. Number of alive and dead animals. Probability of survival.

Excel file 3: Heat shock 210826.xlsx
*C. elegans* strains JU2624 and ERT7...