RNA-seq profiling of Tetep (resistant) and HP2216 (susceptible) rice lines at 0, 48, 72, and 96 hours post infection after M. oryzae inoculation in panicle

Purpose: The aim of this study was to compare transcriptome profiling (RNA-seq) of rice panicle tissue from resistant and suceptible lines at different time intervals infected with M.oryzae. The results were validated using Semi-quantitative PCR. Methods: Panicles from resistant and suceptible rice lines were inoculated with suspension having double distill water, Tween-20 without spores for 0 hour and same suspension with spores was used to inoculate for 48, 72 and 96 hour samples. After collection, samples were frozen on liquid nitrogen, and RNA was extracted using Spectrum™ Plant Total RNA Kit (Sigma). RNA libraries were prepared for sequencing using standard Illumina protocols. Results: Differential Expressed Genes were identified, comparing transcriptome of Control 0 hour vs 48, 72 and 96 hour post infection. Where we get 1070, 2383 and 1080 total significant genes at 48, 72, and 96 hour post infection respectively in HP2216. And, 1564, 1433 and 2263 total significant genes at 48, 72, and 96 hour post infection respectively in Tetep. mRNA was isolated from M.oryzae infected panicle of Tetep (resistant) and HP2216 (Susceptible) rice lines, at 0, 48,72 and 96 hours post infection. After that, mRNA profile was generated by RNA-Seq, in duplicates, using Illumina HiSeq 2000