X-ray crystal structure of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, D98N variant in the presence of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid, UDP-N-acetylglucosamine and UDP at pH 7

X-ray crystal structure of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, D98N variant in the presence of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid, UDP-N-acetylglucosamine and UDP at pH 7 Descriptor: (2~{S},3~{S},4~{R},5~{R},6~{R})-4,5-diacetamido-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxane-2-carboxylic acid, UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase, URIDINE-5'-DIPHOSPHATE, ... Authors: Thoden, J.B, Holden, H.M. Deposit date: 2023-05-25 Release date: 2023-09-13 Last modified: 2023-10-18 Method: X-RAY DIFFRACTION (2.2 Å) Cite: Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis. J.Biol.Chem., 299, 2023嗜热栖热菌（Thermus thermophilus）HB27菌株来源的UDP-2,3-二乙酰胺基-2,3-二脱氧葡糖醛酸-2-差向异构酶D98N突变体，在含有UDP-2,3-二乙酰胺基-2,3-二脱氧葡糖醛酸、UDP-N-乙酰葡糖胺（UDP-N-acetylglucosamine）以及尿苷二磷酸（UDP）、pH值为7的条件下的X射线晶体结构 描述符：(2S,3S,4R,5R,6R)-4,5-二乙酰胺基-6-[[[(2R,3S,4R,5R)-5-[2,4-二氧代嘧啶-1-基]-3,4-二羟基四氢呋喃-2-基]甲氧基-羟基磷酰基]氧基-羟基磷酰基]氧基-3-羟基四氢吡喃-2-羧酸、UDP-2,3-二乙酰胺基-2,3-二脱氧葡糖醛酸-2-差向异构酶、尿苷-5'-二磷酸（URIDINE-5'-DIPHOSPHATE）…… 作者：Thoden J.B.、Holden H.M. 入库日期：2023-05-25 发布日期：2023-09-13 最后修改日期：2023-10-18 实验方法：X射线衍射（分辨率2.2埃） 引用文献：细菌UDP-糖2-差向异构酶的结构分析揭示催化前后的活性位点构象，《生物化学杂志》（J.Biol.Chem.），第299卷，2023年