Expression data from Capsicum annuum inoculated with Fusarium oxysporum Fo47

The colonization of Capsicum annuum roots by Fusarium oxysporum Fo47 induces resistance responses on the plant. Fo47 is a non-pathogenic strain of Fusarium oxysporum. Fo47 colonizes only the most outer layers of the root surface but it does not colonize inner tissues. Pre-treatment of roots with Fo47 reduces the symptom development produced by later pathogen inoculation. The expression of genes in distal tissues was determined by microarray analysis of stems of Fo47-treated plants. Capsicum annuum samples were analyzed using Affymetrix chips of the close-related species Solanum lycopersicum. Samples of Capsicum annuum stems were collected 48 h after inoculation with Fo47 and homogenized in mortar cooled with liquid nitrogen. Total RNA was extracted from the homogenized samples. Total RNA samples were prepared following the protocol of the Affymetrix GeneChip® 3’ IVT Express Kit and then hybridized following the steps of the protocol of the GeneChip® Hybridization, Wash, and Stain Kit. Control plants were inoculated with water (Co) and collected at the same time than Fo47-treated plants (47).