RNAseq analysis of quadriceps muscles from adult mice with constitutively active skeletal and cardiac muscle mTORC1 (through conditional knockout of Tsc1 under the Mck Cre) and their wild type litter mates. Mus musculus

The purpose of this study was to determine how constitutively active mTORC1 signalling alters the transcriptome in skeletal muscle and determine how altered mTORC1 signaling influences the metabolic phenotype. We hypothesized that constitutively active skeletal muscle mTORC1 would lead to increased thermogenic capacity of the muscle and that this promotion of energy expenditure would be protective against diet-indued obesity Overall design: Mice with conditional knockout of the Tsc1 gene were generated. Gastrocnemius muscles were isolated from adult mice n= 28 (7 knockouts and 21 wild types) and total RNA was extracted using TRIzol. Extractions were column purified using a PureLink RNA mini kit and RNA quality was determined using an Agilent bioanalyzer. Sequencing libraries were generated and sent to the UTHSC Molecular Resource Center for RNA sequencing using on AB SOLID 5500 Wildfire.