RNAseq of 6 cell populations along spermatogenesis

Purpose: Analysing gene expression changes along mouse spermatogenesis. Method:The novel method of the isolation of 6 populations of cells at sequential developmental stages -from undifferentiated spermatogonia (Undiff.Spg), differentiating spermatogonia (Diff.Spg), preleptotene (PreL), leptotene/zygotene (LZ), pachytene/diplotene (PD) and round spermatids (RS) was developed. Results: Median sequencing depth was ~18 million reads per sample. Sequencing depth was homogenous across samples in the experiment. We found a profound shift in gene expression between LZ and PD populations. Germ cells mRNA profiles of 6 populations of cells isolated from adult male mice in triplicates.