Data for: Study of the non-clinical healing activities of the extract and gel of Portulaca pilosa L. in skin wounds in wistar rats

Introduction: Portulaca pilosa L., belonging to the family Portulacaceae, is a common herbaceous plant in the Americas and in the Amazon, is popularly known as love-grown and is traditionally used as an aid in the treatment of burns, buds, insect bites and wound healing. This study aims to evaluate the non-clinical topical healing activity of the P. pilosa gel (GPP) and the propyleneglycol extract of P. pilosa (EPP) in Wistar rats.
Methods: For the healing activity, wistar rats were divided into the following groups: negative control (GVE - vehicle, 150 mg/kg), positive control (Fibrinase®- FIB, 100 U/kg), Portulaca pilosa gel 10%, (GPP, 150 mg/kg), and propylenglycollic extract of Portulaca pilosa (EPP, 150 mg/kg), which were submitted to a surgical procedure to obtain the wounds, and were treated topically for 7 days. After treatment, the treated area was removed and a histopathological analysis was performed.
Results: The EPP when analyzed in HPLC was able to identify the presence of gallic acid. EPP significantly modulated the tissue inflammatory response, presenting low number of inflammatory cells in the histopathological study. Treatment with EPP and GPP significantly stimulated angiogenesis and this response was superior to the fibrinase® group. Treatment with EPP and GPP significantly stimulated the proliferation of fibroblasts. The groups treated with EPP and GPP presented an organization pattern of the epidermis and dermis better than the control group, with a mild inflammatory process, with fibroblast proliferation and increased formation of collagen fibers.
Conclusion: Thus, from the results obtained it can be suggested that the phytochemical marker of the P. pilosa species for healing activity is gallic acid and, together with the macroscopic and microscopic findings triggered by the topical applications of EPP and GPP, it can be concluded that this plant species has topical healing activity, with great potential for use, since this pharmacological action is associated with a possible topical anti-inflammatory activity.

引言：毛马齿苋（Portulaca pilosa L.）隶属于马齿苋科（Portulacaceae），是美洲及亚马逊流域常见的草本植物，俗称为"爱生草"，传统上被用作辅助治疗烧伤、皮肤疖肿、虫咬伤及伤口愈合的药物。本研究旨在评估毛马齿苋凝胶（GPP）与毛马齿苋丙二醇提取物（EPP）对Wistar大鼠的非临床局部愈合活性。

方法：为评估愈合活性，将Wistar大鼠分为以下组别：阴性对照组（GVE，赋形剂（vehicle），150 mg/kg）、阳性对照组（纤维酶®（Fibrinase®-FIB），100 U/kg）、10%毛马齿苋凝胶组（GPP，150 mg/kg）以及毛马齿苋丙二醇提取物组（EPP，150 mg/kg）。所有大鼠均接受外科手术构建创伤模型，并连续7天进行局部给药处理。给药结束后，摘取给药区域组织，开展组织病理学分析（histopathological analysis）。

结果：高效液相色谱（High Performance Liquid Chromatography，HPLC）检测显示，EPP中含有没食子酸（gallic acid）。组织病理学分析表明，EPP可显著调节组织炎症反应，炎性细胞浸润数量较低。EPP与GPP给药组均可显著促进血管生成（angiogenesis），且该效果优于纤维酶®对照组。此外，两组均能显著刺激成纤维细胞（fibroblasts）增殖。与对照组相比，EPP与GPP给药组的表皮（epidermis）与真皮（dermis）组织结构更规整，炎症反应更轻微，同时伴随成纤维细胞增殖与胶原纤维（collagen fibers）生成增多。

结论：综上，基于本研究结果可推断，毛马齿苋中与愈合活性相关的植物化学标志物为没食子酸；结合EPP与GPP局部给药后的宏观与微观观测结果，可证实该植物具有局部愈合活性，具备良好的开发应用潜力，其药理作用可能与局部抗炎活性相关。