Verification of RNA-Seq gene expression in abdominal fat of FL and LL cockerels.

Comparisons are shown for 41 DE genes from the RNA-Seq analysis and three additional genes that were not differentially expressed by RNA-Seq analysis, but of interest [NPY, GCGR, and short chicken growth hormone (scGH)]. Fold changes were derived from FL/LL expression ratios of 4 birds/genotype at 7 wk. Positive values correspond to up-regulation in FL chickens, while negative ratios indicate higher expression in abdominal fat of LL cockerels. Pearson correlation analysis showed a high correlation (r = 0.90) and significance (P≤0.001) between the relative expression ratio obtained by the two independent analytical methods (qRT-PCR and RNA-Seq). No significant difference (N.S.) was found for abundance of NPY transcripts between FL and LL chickens by either qRT-PCR or RNA-Seq analyses. The FL and LL expression means and their standard errors are provided in S9 Table for genes verified by qRT-PCR analysis.
Verification of RNA-Seq gene expression in abdominal fat of FL and LL cockerels.