complete de novo assembly and re-annotation of the zebrafish genome

In this project, Iso-Seq was performed on different zebrafish body organs, enabling a comprehensive view of organ-specific transcriptomes. High-quality PacBio Iso-Seq long reads were generated from key zebrafish tissues, including the brain, testis, liver, eye, muscle, ovary, inner ear and kidney, to identify novel isoforms, tissue-specific transcripts, and alternative splicing events. We also made available the Iso-Seq data from embryos from different time points ( hours post fertilization; hpf, 0, 6, 12, and 24). This dataset enhances gene annotation, improves reference genome annotations, and provides insights into zebrafish organ-specific gene expression.