Quantitative Stability of Hematopoietic Stem and Progenitor Cell Clonal Output in Transplanted Rhesus Macaques

Following total body irradiation, four rhesus macaques were transplanted with lentivirally transduced HSPCs containing unique 35 base-pair barcode sequences in the provirus insertions. Blood was extracted from macaques at time points up through 4 years post-transplant and sorted into cell subsets; targeted DNA sequencing of the barcoded region then allowed for quantification of barcodes in cell subsets.