Single cell transcriptomics of the cerebral cortex in mice lacking the PRC2 gene Eed. Single cell transcriptomics of the cerebral cortex in mice lacking the PRC2 gene Eed

The Polycomb Repressive Complex 2 (PRC2) regulates corticogenesis, yet how PRC2 influences cell identity in the mature brain is poorly defined. Using a mouse model in which the PRC2 gene Eed is conditionally deleted from the developing mouse dorsal telencephalon, we performed single nuclei RNA sequencing on the cortical plate of adult heterozygote and homozygote Eed knockout mice compared to controls. Overall design: Using a mouse model in which Eed is conditionally knocked out from all cells derived from the dorsal telencephalon (Emx1-iCre), we performed single nuclei RNA sequencing (snRNA-seq) on the cortical plate of a control (CTRL), a heterozygous (Eed-cHet) knockout, and a homozygous (Eed-cKO) mouse.