Dietary nitrate and nitrite protect against doxorubicin-induced cardiac fibrosis and oxidative protein damage in tumor-bearing mice

Anthracycline-induced cardiotoxicity remains a major limitation in cancer therapy, affecting long-term cardiovascular health in survivors. Dietary nitrate supplementation has shown cardioprotective effects in preclinical models of doxorubicin (Dox)-induced and ischemia-reperfusion injury, but it is unclear whether nitrate and/or nitrite (NOx) would have adverse effects on the anti-cancer efficacy of the drug. To evaluate Dox efficacy against triple negative breast cancer (TNBC) in the presence of dietary nitrate and nitrite, tumor-bearing BALB/c mice (N=5 mice per group, 10 mice total) were treated with 4 weekly intravenous doses of Dox with or without NOx supplementation of their drinking water. Cardiac tissue from the NOx-treated mice exhibited less fibrosis and lower levels of 4-hydroxynonenal-modified proteins, a marker of lipid oxidation and oxidative stress. Tumor sizes varied, but most regressed by the final Dox dose. Importantly, NOx supplementation did not compromise the anti-t..., , # Dietary nitrate and nitrite protect against doxorubicin-induced cardiac fibrosis and oxidative protein damage in tumor-bearing mice

Dataset DOI: [10.5061/dryad.ghx3ffc1p](10.5061/dryad.ghx3ffc1p)

All of these datasets reflect data collected from the 10 study mice (all with breast cancer tumors and doxorubicin treatment, half with nitrite and nitrate supplementation of their drinking water), either during treatment, or after sacrifice.  Data include echocardiography outputs for all mice before and after the treatments as well as measurements of tumor sizes across time of the study, and outcomes of tissue samples collected after sacrifice.  Nitrite and nitrate levels in plasma were analyzed by chemiluminescence, tissue staining and imaging was used to measure cardiac tissue fibrosis and lung tissue metastatic lesions, and cardiac tissue was analyzed by Western blot to evaluate HNE (hydroxynonenal) adducts and Prx3 (peroxiredoxin 3) levels.

## Description of the data and file structur...,

# 膳食硝酸盐与亚硝酸盐可缓解荷瘤小鼠阿霉素诱导的心脏纤维化及氧化蛋白损伤

蒽环类药物诱导的心脏毒性仍是癌症治疗的主要限制因素，会影响幸存者的长期心血管健康。膳食硝酸盐补充在阿霉素（doxorubicin, Dox）诱导损伤及缺血再灌注损伤的临床前模型中已展现出心脏保护作用，但目前尚不清楚硝酸盐和/或亚硝酸盐（NOx）是否会对该药物的抗癌疗效产生不良影响。为评估膳食硝酸盐与亚硝酸盐存在时阿霉素对抗三阴性乳腺癌（triple negative breast cancer, TNBC）的疗效，我们对荷瘤BALB/c小鼠（每组5只，共10只）予以每周静脉注射阿霉素，共4次，同时分别为其饮用水添加或不添加NOx补充剂。经NOx处理的小鼠的心脏组织纤维化程度更低，4-羟基壬烯醛修饰蛋白水平也更低——该蛋白是脂质氧化与氧化应激的标志物。肿瘤大小存在个体差异，但在末次阿霉素给药时多数肿瘤出现退缩。值得注意的是，NOx补充并未削弱抗癌……

数据集DOI: [10.5061/dryad.ghx3ffc1p](10.5061/dryad.ghx3ffc1p)

本数据集所有内容均来源于10只实验小鼠的数据采集结果：所有小鼠均罹患乳腺癌并接受阿霉素治疗，其中半数小鼠的饮用水添加了亚硝酸盐与硝酸盐补充剂，数据采集环节涵盖治疗期间及小鼠处死后两个阶段。采集的数据包括所有小鼠治疗前后的超声心动图结果、研究期间的肿瘤尺寸测量值，以及小鼠处死后收集的组织样本检测结果。亚硝酸盐与硝酸盐在血浆中的水平通过化学发光法进行分析，组织染色与成像技术被用于检测心脏组织纤维化程度及肺组织转移病灶，心脏组织则通过蛋白质印迹法（Western blot）进行分析，以评估HNE（4-羟基壬烯醛，hydroxynonenal）加合物与Prx3（过氧化物还原酶3，peroxiredoxin 3）的表达水平。

数据与文件结构说明……