bacterioplankton communities in the Danjiangkou Reservoir
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A
total of 35 surface-water samples (seven replicates for each sample) were
collected from the Danjiangkou Reservoir on May 1, 2016. Large plankton were
removed by filtering through a 20-μm membrane filter, and bacterioplankton were
collected by filtering through a 0.22-μm-diameter filter. The genomic DNA of
bacterioplankton was extracted from the stored filters using the E.Z.N.A.®
Water DNA Kit (OMEGA, USA) according to the manufacturer's instructions.
Electrophoresis and a NanoDrop ND 2000 (Thermo Scientific, USA) were used to
examine the quantity of the extracted DNA. The V3-V4 region of the bacterial
16S rRNA gene was amplified using 338F and 806R with sample-identifying
barcodes.High-throughput sequencing (Illumina MiSeq PE300 platform, Illumina,
USA) was performed by Shanghai Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai,
China).
创建时间：
2019-09-30
