Genome-wide profiling of expression of human glomeruli and tubules. Genome-wide profiling of expression of human glomeruli and tubules

Glomeruli and tubules are two key components in adult kidney cortex, and are important for kidney function. In order to understand the transcriptional regulation of kidney glomeruli and tubules, we generated genome wide transcription profiles with laser isolated glomeruli and tubules. Human Transcriptome 2.0 GeneChips (HTA2.0) were used to examine global gene expression. Overall design: Five samples of glomeruli or tubules were laser isolated from kidney speciments. RNAs were extracted and prepared for labeling and hybridization on HTA2.0 chips. Data were then normalized and quantified through Affymetrix Transcriptome analysis console (TAC).