PAX5 promotes heterocharomatin formation

Heterochromatin has a high density of DNA and low rates of gene transcription.H3k9me3 is a conserved histone modification, and is best known for its role in constitutive heterochromatin formation.H1155, which is a neuroendocrine large cell lung cancer cell line, has a dense nucleus and a high level of occupancy of H3K9me3. PAX5 is a neurogenesis and B lymphocyte development transcription factor, and endogenous expressed in neuroendocrine carcinoma, including H1155 cell line. To assess whether PAX5 promotes heterochromatin formation in H1155, we knock out PAX5 in H1155 cell (KO-PAX5) by CRISPR/Cas9 and use H3K9me3 to performed ChIP-seq. ChIP-seq analysis of H1155-WT (control) and KO-PAX5 (experimental group) with H3K9me3 antibody, two replicates. In order to calibrate the data, we mix 1% of Drosophila Schneider 2 (S2) cells with the tested cells and perform sonication, sipike in antibosy were added at the same time with H3K9me3.