Absolute protein quantification allows differentiation of cell specific metabolic routes and functions.

Data from ProteomeXchange, PXD ID: PXD001352. Experiment: mouse, brain, file: 20140327_EXQ3_JRW_D75_L2.mzml. Published as part of Proteomics. 2014 Dec 5 . From the Abstract: {{i}} Total protein approach (TPA) is a proteomic method which allows calculation of concentrations of individual proteins and groups of functionally related proteins in any protein mixture without spike-in standards. Using the two step digestion-filter aided sample preparation method and LC-MS/MS analysis we generated comprehensive quantitative datasets of mouse intestinal mucosa, liver, red muscle fibers, brain, and of human plasma, erythrocytes, and tumor cells lines. We show that the TPA based quantitative data reflect well-defined and specific physiological functions of different organs and cells, for example nutrient absorption and transport in intestine, amino acid catabolism and bile secretion in liver, and contraction of muscle fibers ... {{/i}}