Transcriptional profiling to compare genes induced by CYTL1 to genes triggered by VEGF-A under normoxic and hypoxic conditions. Homo sapiens

CYTL1 is a bona fide pro-angiogenic factor produced by endothelial colony forming cells (ECFCs), which mediates high angiogenic sprouting of ECFCs and vessel wall endothelial cells by autocrine and paracrine stimulation, respectively. To evaluate whether the signaling response to CYTL1 mimics the response to VEGF-A or induces unique signaling pathways in endothelial cells, we performed transcriptional profiling to compare the gene repertoire induced by CYTL1 to the repertoire triggered by VEGF-A in HUVEC under standard normoxic conditions or angiogensis stimulating hypoxic conditions. Overall design: Each experimental treatment condition was conducted in triplicates. After RNA isolation triplicates were pooled and subjected to cRNA synthesis. Untreated HUVECs were used as reference.