miR-seq of human frontal lobe

Two normal frontal white matter samples, obtained from two pediatric patients (A boy of 3 year old and a girl of 3 mount old) undergoing focal brain resection for head injury, were obtained. miRVana miRNA Isolation Kit (Ambion, USA) was used following the manufacturing instruction to isolate miRNA from these two samples. Brain samples were collected during neurosurgery of the patients at the Policlinico Gemelli in Rome. The study was approved by the local ethical committee. The miRNA was sequenced using Illumina HiSeq2000 machine.