Transcriptomic analysis of evolved strains.

We develop a CRISPR-Cas assisted random mutation (CARM) technology for whole genome mutagenesis. As a proof-of-principle, CARM was applied to evolve the capacity of Saccharomyces cerevisiae to produce β-carotene. Thees files are transcriptomic analysis of finally evolutionary strain C7-143, processed evolutionary strain C3-06 and C5-63, and the original strains C0 and BY47419.我们开发了一种用于全基因组诱变的CRISPR-Cas辅助随机突变（CARM）技术。作为原理验证研究，我们利用该技术进化酿酒酵母（Saccharomyces cerevisiae）以提升其合成β-胡萝卜素（β-carotene）的能力。本数据集收录的文件对应最终进化菌株C7-143、处理后的进化菌株C3-06与C5-63，以及原始菌株C0和BY47419的转录组分析数据。