FRET analysis of subunit protein release triggered by ATP and GroES.

Fluorescence intensities at wavelengths greater than 420et al [20]. Gray trace indicates changes in FRET fluorescence in the presence of ATP only (no GroES), green trace indicates FRET changes in the presence of ATP and equimolar GroES. B. FRET traces for mutants. Black trace indicates SR-1, and Gray trace indicates D398A-RW. Note the differences in time scale between panels A and B.

在波长大于420纳米处的荧光强度，如et al [20]所述。灰色轨迹表示仅在ATP存在下（无GroES）的FRET荧光变化，绿色轨迹表示在ATP和等摩尔GroES存在下的FRET变化。图B展示了突变体的FRET轨迹。黑色轨迹代表SR-1，灰色轨迹代表D398A-RW。请注意图A和图B在时间尺度上的差异。