Deep sequencing of yeast and mouse tRNAs and tRNA fragments using OTTR

Deep sequencing of tRNAs have historically been notoriously difficult. Here, we benchmark a newly developed library prep protocol termed OTTR against intact tRNAs as well as tRNA fragments and show that OTTR outperforms any other commercial cloning protocol. Intact tRNA from mouse testis and S. cerevisiae, as well as tRNA fragments (tRFs) from mouse cauda epididymis and S. cerevisiae were cloned using three different protocols. Results were then compared to published data generated using different cloning protocols.