Casuarina

Chromosome-scale de novo genome assembly andannotation of three representative Casuarina species:C. equisetifolia, C. glauca, and C. cunninghamianaAustralian pine (Casuarina spp.) is extensively planted in tropical and subtropical regions for wood produc?tion, shelterbelts, environmental protection, and ecological restoration due to their superior biological char?acteristics, such as rapid growth, wind and salt tolerance, and nitrogen fixation. To analyze the genomicdiversity of Casuarina, we sequenced the genomes and constructed de novo genome assemblies of thethree most widely planted Casuarina species: C. equisetifolia, C. glauca, and C. cunninghamiana. We gener?ated chromosome-scale genome sequences using both Pacific Biosciences (PacBio) Sequel sequencing andchromosome conformation capture technology (Hi-C). The total genome sizes for C. equisetifolia, C. glauca,and C. cunninghamiana are 268 942 579 bp, 296 631 783 bp, and 293 483 606 bp, respectively, of which25.91, 27.15, and 27.74% were annotated as repetitive sequences. We annotated 23 162, 24 673, and 24 674protein-coding genes in C. equisetifolia, C. glauca, and C. cunninghamiana, respectively. We then collectedbranchlets from male and female individuals for whole-genome bisulfite sequencing (BS-seq) to explore theepigenetic regulation of sex determination in these three species. Transcriptome sequencing (RNA-seq)revealed differential expression of phytohormone-related genes between male and female plants. In sum?mary, we generated three chromosome-level genome assemblies and comprehensive DNA methylation andtranscriptome datasets from both male and female material for three Casuarina species, providing a basisfor the comprehensive investigation of genomic diversity and functional gene discovery of Casuarina in the future.. Please cite following reference if you use this dataset in your study:Zhang, Y., Wei, Y., Meng, J., Wang, Y., Nie, S., Zhang, Z., ... &amp; Gu, L. (2023). Chromosome‐scale de novo genome assembly and annotation of three representative Casuarina species: C. equisetifolia, C. glauca, and C. cunninghamiana. <i>The Plant Journal</i>, <i>114</i>(6), 1490-1505.All suggestions are welcome to [e-mail]