SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in July 2014

Flow cytometry data was collected in July 2014, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术（Flow Cytometry）数据采集于2014年7月，采集区域为澳大利亚南部近海海域。本研究的核心目标为构建澳大利亚南部大陆架海域生态系统的基础背景资料，阐明上升流/高盐径流事件对微生物群落的影响，最终开发该区域的生物地球化学模型。采样工作依托南澳大利亚综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目，在“恩格林号”（RV Ngerin）科考船的巡航任务中完成。每一次巡航中，科研人员均对叶绿素荧光最大值层（Deep Chlorophyll Maximum, DCM）的物理、化学及生物学特性开展调查。本研究采集了超微型浮游植物、细菌与病毒的流式细胞术数据。本研究期间共完成6个主要采样站位的样品采集：其中5个站位位于100米等深线海域，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）与SAM5CB/B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），位于袋鼠岛西南海域。需注意的是，结合站位间距（14~25海里）、陆架中层海流平均流速分量（0.01 m·s⁻¹）与科考船平均航速（约9节）的测算结果，各站位所采集的水体均属于不同水团。此外，研究团队偶尔会从袋鼠岛国家参考站（National Reference Station, NRS，35.832°S，136.447°E）以及发生高盐径流的斯宾塞湾口锚站（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E）采集补充样本。