5-hydroxytryptamine synthesized in the Aorta-Gonad-Mesonephros regulates hematopoietic stem and progenitor cell survival

Whole-genome gene expression analysis was performed using the AGMs from two littermate pairs of tph2+/+, tph2+/- or tph2-/- at E11.5. The total RNA was extracted using TRNzol (Tiangen, Beijing) and cDNA samples were sequenced using Illumina HiSeq3000 (RiboBio Co. Ltd, Guangzhou). The reference Mus musculus genome and gene information were downloaded from the National Center for Biotechnology Information Database (NCBI: http://www.ncbi.nlm.nih.gov/). Examination of gene expression levels in the AGMs of tph2+/+, tph2+/- or tph2-/- at E11.5本研究以胚胎发育第11.5天（E11.5）的2对同窝tph2+/+、tph2+/-及tph2-/-小鼠的主-性腺-中肾区（aorta-gonad-mesonephros，AGM）为材料，开展全基因组基因表达分析。总RNA采用TRNzol试剂（天根生化科技有限公司，北京）提取，cDNA样本使用Illumina HiSeq3000测序平台（广州锐博生物科技有限公司）完成测序。小家鼠（Mus musculus）参考基因组及基因注释信息下载自美国国家生物技术信息中心（National Center for Biotechnology Information，NCBI）数据库，网址为http://www.ncbi.nlm.nih.gov/。本研究对胚胎发育第11.5天的tph2+/+、tph2+/-及tph2-/-小鼠主-性腺-中肾区的基因表达水平进行了检测分析。