Vacuolar transport and function of <em>Saccharomyces cerevisiae</em> sterol ester hydrolase Tgl1

This dataset supports the thesis titled "Vacuolar transport and function of Saccharomyces cerevisiae sterol ester hydrolase Tgl1." It includes results from microscopic analyses, thin-layer chromatography, and Western blotting of wild-type and mutant strains, as well as the corresponding statistical analyses. For fluorescence microscopy, images were acquired as single optical mid-sections at the approximate midpoint of the cells, without Z-stack collection. The vacuolar membrane was visualized using Vph1-EGFP. Vacuolar membrane microdomains were observed as discontinuous patterns of fluorescence signals on the vacuolar membrane. The localization of Tgl1 was visualized using Tgl1-mCherry. For statistical analysis, 30 cells were analyzed per field in three independent microscopic fields for each strain. Thin-layer chromatography data were also statistically analyzed based on results from three independent experiments. This dataset focus on the localization and function of Tgl1 after a diauxic shift.