Heatwaves exacerbate pollen limitation through reductions in pollen production and pollen vigour

Background and aims
Increasingly frequent heat waves threaten the reproduction of flowering plants; compromising the future persistence, adaptive capacity, and dispersal of wild plant populations, and also the yield of fruit-bearing crop plants. Heat damages development of sensitive floral organs and gametes, which inhibits pollen germination, pollen tube growth, and fertilization. However, the role of heat has not been integrated into the framework of pollen quantity and quality limitation and how heat influences the success of cross and self-pollination.
Methods
We exposed developing flowers to either control temperature (25C:20C) or extreme heat (35C:20C) over 72 hours. We then hand pollinated them with either crossed or self-derived pollen from the same temperature treatment to determine direct and interactive effects of simulated heatwaves on pollen tube growth and resulting seed set. We also collected anthers from virgin flowers to measure heat impacts pollen production. 
Key resu..., , , # Heatwaves exacerbate pollen limitation through reductions in pollen production and pollen vigour

[https://doi.org/10.5061/dryad.tmpg4f572](https://doi.org/10.5061/dryad.tmpg4f572)

## Description of the data and file structure

Provides all information associated with the data and analyses of the publication \"Heatwaves exacerbate pollen limitation through reductions in pollen production and pollen vigour\" published in AoB Plants

### Files and variables

#### File: aopb\_anthers.csv

**Description:** contains all data used for analysis of pollen production

##### Variables

* t_num: Treated cohort number plant was in for temperature treatment
* p_num: Number given to plant from temperature treat
* p_trt: Plant temperature treatment (Control or Heat; 25° Celsius or 35° Celsius)
* start_wgt: weight of vial before sampling 3ml (weight in grams)
* vial_wgt: weight of vial used when empty (weight in grams)
* final_wgt: weight after sampling 3ml (weight in grams)
* pollen_grains: average n...