Hypersensitiveness of cup-1(gk245) animals to cholesterol availability.
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Wild-type (N2) and cup-1 mutant animals (gk245) were grown with cholesterol supplementation or in low-cholesterol conditions and phenotypes were evaluated in F2 progeny. For total offspring, embryos and larvae were quantified from individual nematodes for six consecutive days. Sperm and oocyte numbers were obtained from DAPI stained gonads. Embryo lethality is defined as the percentage of non-hatched embryos after 24 h. Larval development is defined as the number of adults divided by the total number of L1 larvae. Dauer percentage was evaluated by larvae survival to SDS treatment after 15 days of growth at 25°C. For Offspring, Embryo Lethality and Larval Development, n>70 from three independent determinations. For Sperm and Oocyte Number, n>40. Dauer, n>1500.*p
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2015-12-02
