Effect of knockdown of GRIK1 on gene expression dring glioblastoma progression

Glioblastoma (GBM) is the most aggressive cancer in brain and contains a high mortality rate due to lack of effective treatment strategy. Molecular mechanisms of GBM characteristic invasive growth are urgently need to improve the poor prognosis.Here we reported that knockdown of GRIK1 retarded GBM cells growth and invasion.Moreover,high levels of GRIK1 were correlated to poor prognosis of GBM, consistent with The Cancer Genome Atlas (TCGA) database. Taken together, our study identified that GRIK1 is a novel and vital factor of GBM progression and may serve as a new biomarker for prognosis and therapy of GBM patients. Overall design: To investigate the downstream mechanism of GRIK1 in GBM progression, we established U87-MG cells in which GRIK1 was knockdown by shRNA.We then performed gene-expression profile analysis using data obtained from RNA-seq of these cells. Comparative gene expression profiling analysis of RNA-seq data for U87-MG and its KD derivative.