Summary of phenotypes of Set1+ cells, set1Δ cells and Set1 mutants.

aFor details of classification, see text; partial func/null, partial function with phenotypes more similar to set1Δ cells; partial func/silent, partial function with phenotypes more similar to Set1+ cells.bAmino acid substitution in Set1.cAverage levels +/− range of H3K4me1, H3K4me2 and H3K4me3 measured in whole cell extracts from Set1 mutants by quantitative western blotting (n = 2); values are normalized to the levels measured in whole cell extracts from a wild type Set1+ strain. No value for +/− range is given if the two measurements were identical. See Figure 3 and text for details.dPol II gene silencing at the rDNA was assessed by measuring the level of Ty1his3AI mRNA/PYK1 mRNA in total RNA relative to that from a Set1+ strain where the ratio of Ty1his3AI/PYK1 was set to 1. The values shown are average +/− SD for n repeats.eSuppression of ipl1-2 growth defects at the restrictive temperature (30°C) was measured in growth assays described in the text and shown in Figure 5. Yes, growth at 30°C; No, severely reduced growth at 30°C; partial-yes, slightly reduced growth at 30°C; partial-no, intermediate reduction of growth at 30°C.