Myelin like electrogenic filamentation and Liquid Microbial Fuel Cells Dataset overview.

Biofilm at water-oil interface of hypoxic water columns of microcosms, prepared from a lacustrine sample, that used diesel as a carbon source was found to show electrogenic properties. These microcosms named, liquid microbial fuel cells (L-MFCs) were electrically characterized using a custom electronic analyzer; accurate determination of voltage (V), power density (W/m 2), and current density (A/m2) for both charge and discharge phases was carried out. The instrument made it possible to carry out cell characterizations using resistive loads between 0 Ω (Ohm) and 10 kΩ. During the hypoxic and electrogenic phase, the synthesis of a system of “bacterial piping induction”, produced filaments of hundreds of micrometers in which the microbial cells are hosted. Ultrastructural microscopy collected by scanning (SEM), transmission (TEM), immunofluorescence, Thunder Imager 3D, confocal laser scanning (CLSM) microscopy revealed a “myelin like“ structure during filamentation processes; this “myelin like” structure exhibited cross-reactivity towards different epitopes of the myelin basic protein (MBP) and Claudin 11 (O4) of human oligodendrocytes. The disclosure of these filamentation processes could be helpful to describe further unconventional microbial structures in aquatic ecosystems and of the animal world.

在水柱微域缺氧水层中，从湖泊样品中制备的以柴油为碳源的生物膜，其于水-油界面的水柱中表现出电生成特性。这些被称为液体微生物燃料电池（L-MFCs）的微域，通过定制电子分析仪对其进行了电学表征；对充放电两相的电压（V）、功率密度（W/m²）和电流密度（A/m²）进行了精确测定。该仪器使得在0 Ω至10 kΩ的电阻负载之间进行细胞表征成为可能。在缺氧和电生成阶段，'细菌管道诱导'系统的合成产生了数百微米的菌丝，其中微生物细胞得以栖居。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、免疫荧光、Thunder Imager 3D、共聚焦激光扫描显微镜（CLSM）收集的超微结构图像揭示了在菌丝形成过程中的'髓鞘样'结构；这种'髓鞘样'结构与人类少突胶质细胞的髓鞘基本蛋白（MBP）和Claudin 11（O4）的不同表位表现出交叉反应性。这些菌丝形成过程的揭示，有助于进一步描述水生生态系统及动物界中非传统的微生物结构。