Synergistic effect of hydralazine associated with triazoles on Candida spp. in planktonic cells - Supplementary material Table

<b>Objective: </b>To evaluate the antifungal activity of hydralazine hydrochloride alone and in synergy with azoles against <i>Candida </i>spp. and the action mechanism. <b>Methods: </b>We used broth microdilution assays to determine the MIC, checkerboard assays to investigate synergism, and flow cytometry and molecular docking tests to ascertain action mechanism. <b>Results: </b>Hydralazine alone had antifungal activity in the range of 16–128 μg<b>/</b>ml and synergistic effect with itraconazole versus 100% of the fungal isolates, while there was synergy with fluconazole against 11.11% of the isolates. There was molecular interaction with the receptors exo-B(1,3)-glucanase and CYP51, causing reduced cell viability and DNA damage. <b>Conclusion: </b>Hydralazine is synergistic with itraconazole and triggers cell death of <i>Candida </i>spp. at low concentrations, demonstrating antifungal potential.