Spleen transcriptomes during behavioural fever in Salmo salar

The transcriptomic analysis was evaluated using RNA-seq of salmon challenged with the birnavirus IPNv under two different thermal conditions: (a) constant temperature (mean temperature 15 ± 0.9 ºC, “No-Fever”) and (b) temperature gradient (mean temperature 15 ± 7.4 ºC, “Fever”). In parallel, fish in another tank were treated by adding 100 ml virus free cell culture supernatant to the water (mean temperature 15 ± 7.4 ºC, "mock-infected"). We identified a specific transcriptomic signature for each group in the RNA-seq data and identifying a set of key components that control inflammatory modulation during behavioural fever upon viral infection in mobile ectotherms. Spleen mRNA profiles were generated by deep sequencing, in triplicate, using Illumina MiSeq. Tisue were obtained from parr Salmo salar at 24, 48, 72- and 96-hours post infection with IPNv and under two different thermal conditions: (a) constant temperature (mean temperature 15 ± 0.9 ºC, “No-Fever”) and (b) temperature gradient (mean temperature 15 ± 7.4 ºC, “Fever”). In parallel, another spleen samples were obtained from salmon exposed at virus-free cell culture supernatant and temperature gradient (mean temperature 15 ± 7.4 ºC, “Mock”).