Comprehensive Analysis of Mouse Hippocampal Lysine Acetylome Mediated by Sea Cucumber Peptides Preventing Memory Impairment

Memory impairment is becoming a potential
health issue with the
delicacy of diet and social stress. Sea cucumber peptides (SCP) prevent
memory impairment, as previously reported. In this study, further
research was performed using hippocampal lysine-acetylome to explore
molecular regulation mechanisms. C57BL/6 mice were treated with scopolamine
via intraperitoneal injection to simulate memory impairment. To determine
the influence of SCP on the total acetylated-protein level of the
hippocampus, acetylated-proteomics was performed. SCP increased the
acetylation level of histone (H3 and H4). Meanwhile, for non-histones,
the differentially acetylated proteins were involved in multiple memory-related
pathways, as shown by KEGG enrichment analysis. Additionally, long-term
potentiation was confirmed by western blotting. Finally, a combined
analysis of proteome and lysine acetylome revealed that SCP contributed
to synaptic vesicle cycle regulation and dopamine metabolism. Consequently,
our findings revealed that SCP was potentially neuroprotective by
regulating post-transcriptional hippocampal protein acetylation.