Genetic regulation and targeted reversal of lysosomal dysfunction and inflammatory sterol metabolism in pulmonary arterial hypertension

We treated human pulmonary artery endothelial cells (PAECs) with silencing RNA against the nuclear receptor coactivator 7 (NCOA7) or negative control (NC). We further treated the cells to either control or IL-1B conditions (1ng/mL) for 24 hours. We created four groups of cells in triplicate: (1) si-Negative Control (NC) PAECs (2) siNC+IL1B PAECs (3) siNCOA7 PAECs (4) siNCOA7+IL1B PAECs