PFOS perturbs mitochondrial metabolism in skeletal muscle in vivo

The purpose of this project was to investigate the effects of PFOS on skeletal muscle in vivo. To perform this work, Cyp2b-null and hCYP2B6-Tg mice were treated with 0, 1, or 10 mg/kg/day PFOS for 21 days. Gastrocnemius muscle was collected during necropsy and various assays were performed. RNA seq was performed to determine what gene expression changes occured due to PFOS treatment and the difference between genotypes. Overall design: Male and female Cyp2b-null and hCYP2B6-Tg mice (n = 3-5) were treated with 0, 1, or 10 mg/kg/day PFOS via oral gavage for 21 days. Mice were euthanized via carbon dioxide asphyxiation followed by heart puncture. Gastrocnemius muscle was collected and snap frozen in liquid nitrogen.