Tea Grape Reduces Abdominal Aortic Occlusion-Induced Lung Injury

Abstract Introduction: Ischemia-associated mortality caused by aortic cross-clamps, as in ruptured abdominal aorta aneurysm surgeries, and reperfusion following their removal represent some of the main emergency conditions in cardiovascular surgery. The purpose of our study was to examine the potential protective effect of tea grape against aortic occlusion-induced lung injury using biochemical, histopathological, immunohistochemical, and quantitative analyses. Methods: Thirty-two male Sprague-Dawley rats were randomly assigned into four groups: control (healthy), glycerol + ischemia/reperfusion (I/R) (sham), I/R, and I/R + tea grape. Results: Following aortic occlusion, we observed apoptotic pneumocytes, thickening in the alveolar wall, edematous areas in interstitial regions, and vascular congestion. We also observed an increase in pulmonary malondialdehyde (MDA) levels and decrease in pulmonary glutathione (GSH). However, tea grape reduced apoptotic pneumocytes, edema, vascular congestion, and MDA levels, while increased GSH levels in lung tissue. Conclusion: Our findings suggest that tea grape is effective against aortic occlusion-induced lung injury by reducing oxidative stress and apoptosis.

引言：腹主动脉瘤破裂手术中使用主动脉阻断钳引发的缺血相关死亡，以及移除阻断钳后的再灌注损伤，均为心血管外科的主要急症之一。本研究旨在通过生化、组织病理学、免疫组织化学及定量分析，探究茶葡萄对主动脉阻断诱导肺损伤的潜在保护作用。方法：将32只雄性Sprague-Dawley（SD）大鼠随机分为四组：对照组（健康大鼠）、甘油+缺血/再灌注（I/R）组（假手术组）、I/R组以及I/R+茶葡萄组。结果：主动脉阻断后，我们观察到肺泡上皮细胞凋亡、肺泡壁增厚、间质区域水肿以及血管充血；同时肺组织丙二醛（malondialdehyde，MDA）水平升高，谷胱甘肽（glutathione，GSH）水平降低。茶葡萄干预可减少肺泡上皮细胞凋亡、水肿及血管充血，降低MDA水平，并提升肺组织中GSH的含量。结论：本研究结果表明，茶葡萄可通过减轻氧化应激与细胞凋亡，有效改善主动脉阻断诱导的肺损伤。