Structure of the D10N mutant of beta-phosphoglucomutase from Lactococcus lactis trapped with native reaction intermediate beta-glucose 1,6-bisphosphate to 2.2A resolution.

Structure of the D10N mutant of beta-phosphoglucomutase from Lactococcus lactis trapped with native reaction intermediate beta-glucose 1,6-bisphosphate to 2.2A resolution. Descriptor: 1,3-PROPANDIOL, 1,6-di-O-phosphono-beta-D-glucopyranose, Beta-phosphoglucomutase, ... Authors: Robertson, A.J, Bisson, C. Deposit date: 2017-07-25 Release date: 2018-08-29 Last modified: 2024-01-17 Method: X-RAY DIFFRACTION (2.15 Å) Cite: van der Waals Contact between Nucleophile and Transferring Phosphorus Is Insufficient To Achieve Enzyme Transition-State Architecture Acs Catalysis, 2018

乳酸乳球菌（Lactococcus lactis）来源的β-磷酸葡萄糖变位酶（beta-phosphoglucomutase）D10N突变体的晶体结构，该结构捕获了天然反应中间体β-葡萄糖1,6-二磷酸（beta-glucose 1,6-bisphosphate），解析分辨率达2.2埃（Å）。相关配体描述符：1,3-丙二醇（1,3-PROPANDIOL）、1,6-二-O-膦酰基-β-D-吡喃葡萄糖（1,6-di-O-phosphono-beta-D-glucopyranose）、β-磷酸葡萄糖变位酶（Beta-phosphoglucomutase）…… 作者：Robertson, A.J.、Bisson, C. 沉积日期：2017-07-25 发布日期：2018-08-29 最后修改日期：2024-01-17 实验方法：X射线衍射（X-RAY DIFFRACTION），实际解析分辨率为2.15埃（Å）。引用文献：《亲核试剂与转移磷原子之间的范德华接触不足以实现酶过渡态结构》（van der Waals Contact between Nucleophile and Transferring Phosphorus Is Insufficient To Achieve Enzyme Transition-State Architecture），发表于《ACS催化》（Acs Catalysis）2018年。