single-cell circadian rhythm in live zebrafish larva

The files (in tiff format) are 3D reconstructions at different time points for each individual fish, aligned using CMTK toolkits except for Supplementary Movie 3.
Supplementary Movie 1&2. The combined image stacks of the whole brain using two-photon imaging . The fish was imaged from 3.5 dpf to 7.5 dpf every 12 hours (9 stacks). The fish was raised under LD condition.
Supplementary Movie 3. Confocal 3D reconstructions of zebrafish pineal gland. Zebrafish larvae was co-labeled with nr1d1:VNP (blue) and aanat2:mRFP (red) .
Supplementary Movie 4-9. The combined image stacks of the pineal gland using two-photon imaging under LD condition (LD fish1-6). The fish was imaged from 3.5dpf to 6.5dpf every 12 hours (7 stacks).
Supplementary Movie 10-13. The combined image stacks of the pineal gland using two-photon imaging under DD condition (DD fish 1-4). The fish was imaged from 3.5dpf to 6.5dpf every 12 hours (7 stacks).
Supplementary Movie 14&15 (LD fish 7&8). The combined image stacks of the pineal gland using two-photon imaging under LD condition. The fish was imaged at 5.0 dpf in every two hours (12 stacks).
Supplementary Movie 16-18 (DD fish 5-7). The combined image stacks of the pineal gland using two-photon imaging under DD condition. The fish was imaged at 5.0 dpf in every two hours (12 stacks).

本数据集的文件均为TIFF格式（TIFF），包含每条斑马鱼不同时间点的3D重建结果；除补充视频3外，所有数据均通过CMTK工具包（CMTK）完成对齐。
补充视频1与2：采用双光子成像（two-photon imaging）获取的全脑组合图像栈。该斑马鱼的成像周期为受精后3.5天至7.5天（受精后天数，dpf），每12小时采集一次，共获得9组图像栈。实验中斑马鱼饲养于光暗周期（LD）条件下。
补充视频3：斑马鱼松果体的共聚焦3D重建结果。该斑马鱼幼虫同时被nr1d1:VNP（蓝色）与aanat2:mRFP（红色）标记。
补充视频4至9：光暗周期条件下采用双光子成像获取的斑马鱼松果体组合图像栈（对应LD组鱼1至6）。该批次斑马鱼的成像周期为受精后3.5天至6.5天，每12小时采集一次，共获得7组图像栈。
补充视频10至13：持续黑暗条件下采用双光子成像获取的斑马鱼松果体组合图像栈（对应DD组鱼1至4）。该批次斑马鱼的成像周期为受精后3.5天至6.5天，每12小时采集一次，共获得7组图像栈。
补充视频14与15（对应LD组鱼7与8）：光暗周期条件下采用双光子成像获取的斑马鱼松果体组合图像栈。该批次斑马鱼在受精后5.0天开始成像，每2小时采集一次，共获得12组图像栈。
补充视频16至18（对应DD组鱼5至7）：持续黑暗条件下采用双光子成像获取的斑马鱼松果体组合图像栈。该批次斑马鱼在受精后5.0天开始成像，每2小时采集一次，共获得12组图像栈。