Splenic fibroblasts control marginal zone B cell movement and function via two distinct Notch2-dependent regulatory programs. Allman et al.

This dataset provides supporting material for the manuscript “Splenic fibroblasts control marginal zone B cell movement and function via two distinct Notch2-dependent regulatory programs”. Using genetic and pharmacological approaches we investigated the role of Notch signaling in splenic marginal zone B (MZB) cell homeostasis in mouse models. Combining data generated through flow cytometry, confocal imaging, and RNAseq, we identified stromal sources of Delta-Like1 Notch ligands within splenic white pulp follicles and distinguished between Myc-dependent and Myc-independent branches of the Notch regulated transcriptome within MZB cells. The following dataset represents the confocal images generated in transgenic mouse models, as well as the cell proportions and absolute numbers of cells generated and calculated through flow cytometry.

本数据集为学术论文《脾脏成纤维细胞通过两种Notch2依赖性调控程序调控边缘区B细胞运动与功能》提供支撑材料。本研究借助遗传学与药理学手段，在小鼠模型中探究了Notch信号通路（Notch signaling）在脾脏边缘区B细胞（marginal zone B cell, MZB）稳态维持中的作用。本研究整合流式细胞术（flow cytometry）、共聚焦成像（confocal imaging）与RNA测序（RNAseq）所获数据，在脾脏白髓滤泡中鉴定出Delta样1 Notch配体（Delta-Like1 Notch ligands）的基质细胞来源，并区分了MZB细胞内Notch调控转录组（transcriptome）中Myc依赖性与Myc非依赖性分支。本数据集包含转基因小鼠模型中获取的共聚焦成像结果，以及通过流式细胞术生成与计算得到的细胞比例与细胞绝对计数。