Sensitivity and response of Macquarie Island plants to fuel contaminants (germination and early growth endpoints)

Seeds of twelve plant species were collected from Macquarie Island during January and February 2013. The seeds were used in a series of laboratory-based tests during 2013, at the University of Wollongong. Tests were conducted to determine species suitably for use in laboratory-based toxicity testing, using germination success and seedling growth (shoot and root length) as end points. Two soil types (containing low and high organic carbon contents) were used, to reflect the variable organic carbon content found in soils at Macquarie Island. Test soils were spiked with Special Antarctic Blend (SAB) diesel fuel and aged for 14 days to generate a concentration series of SAB-contaminated soils. Exposure doses were quantified as the concentration of total petroleum hydrocarbons (TPH, nC9-nC18) on a soil dry mass basis. Based on germination success in initial trials, four species (Colobanthus muscoides Hook.f., Deschampsia sp., Epilobium pendunculare A.Cunn. and Luzula crinita Hook.f.) were included in definitive toxicity tests. Description of files provided: - FILE: GabriellaMacoustra_Germination.xlsxExcel data file providing results of germination trials. Includes test development data (germination response of test species on test substrates without addition of fuel toxicants). - FILE: GabriellaMacoustra_Growth.xlsxExcel data file providing results of germination trials. Provides measurements from image analysis of roots and shoots of plants used in germination trials. - FILE: GabriellaMacoustra_Soil Characterisation.xlsxExcel data file providing soil characterisation data for the two soil substrates (sandy and peaty) used in the germination trials. Includes spiked fuel concentrations and a range of physicochemical properties. - FILE: Macoustra 2013_Thesis.pdfPDF file of University of Wollongong Honours thesis submitted by Gabriella Macoustra 2013. - FILES (Field and lab notebook scans): Notebook-Field_42420.pdf, Notebook-Field_42422.pdf, Notebook-Field_42398.pdf, Notebook-Lab_101036.pdf. Four scanned PDF files of field notes taken by Corrine de Mestre, related to seed collections on Macquarie Island during 2012/13.

2013年1-2月，研究人员从麦夸里岛（Macquarie Island）采集了12种植物的种子。上述种子于2013年在伍伦贡大学（University of Wollongong）开展了一系列室内实验。实验旨在评估物种用于室内毒性测试的适用性，以萌发成功率及幼苗生长（地上部与根长）作为观测终点。实验设置两种有机碳含量梯度的土壤类型，以反映麦夸里岛土壤中有机碳含量的实际变异情况。供试土壤经南极专用混合柴油（Special Antarctic Blend, SAB）污染处理后老化14天，以此构建SAB污染土壤的浓度梯度。暴露剂量以土壤干重计的总石油烃（total petroleum hydrocarbons, TPH，nC9-nC18）浓度进行量化。根据初始试验的萌发结果，最终选取4个物种开展确定性毒性测试，分别为：漆姑草属*Colobanthus muscoides* Hook.f.、发草属*Deschampsia* sp.、长柄柳叶菜*Epilobium pendunculare* A.Cunn.以及地杨梅属*Luzula crinita* Hook.f.。
本次提供的文件说明如下：
- GabriellaMacoustra_Germination.xlsx：该Excel数据文件包含萌发试验结果，涵盖试验开发阶段数据（即未添加燃油有毒物的供试基质上供试物种的萌发响应情况）。
- GabriellaMacoustra_Growth.xlsx：该Excel数据文件包含萌发试验结果，提供了萌发试验中植株根、茎部的图像分析测量数据。
- GabriellaMacoustra_Soil Characterisation.xlsx：该Excel数据文件包含萌发试验所用两种基质（砂质土与泥炭土）的土壤表征数据，涵盖燃油加标浓度及一系列理化性质参数。
- Macoustra 2013_Thesis.pdf：Gabriella Macoustra于2013年提交至伍伦贡大学（University of Wollongong）的荣誉学士学位论文PDF文件。
- 野外与实验室笔记扫描件：Notebook-Field_42420.pdf、Notebook-Field_42422.pdf、Notebook-Field_42398.pdf、Notebook-Lab_101036.pdf。共4份扫描PDF文件，为Corrine de Mestre于2012/2013年在麦夸里岛采集种子期间记录的野外笔记。