ChIP-seq of Mycobacterium smegmatis PafBC to identify in vivo binding sites. ChIP-seq of Mycobacterium smegmatis PafBC to identify in vivo binding sites

We aimed to determine the binding sites of the putative transcriptional regulator PafBC under DNA damage stress induced by mitomycin C or under oxidative stress induced by hydrogen peroxide. Therefore, we chose a ChIP-seq approach, crosslinking cells before PafBC-DNA complexes were immunoprecipitated with a PafBC-specific antibody.