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<b>Bourne </b><b><i>et al., </i></b><b>Aphid parasitism alters induced plant responses allowing a hyperparasitoid to locate its hidden parasitoid host. </b>Annotated EPG files from the electropenetrography (EPG) experiment to study the feeding behaviour of (parasitised) aphids as depicted in Figure 6.

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DataCite Commons2025-11-05 更新2026-02-09 收录
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To investigate the feeding behaviour of (un)parasitised B. brassicae and M. persicae aphids, the electrical penetration graph (EPG) technique was employed. In short, a golden wire (18 μm diameter and 1.5 ± 0.5 cm in length) was gently attached to the dorsum of apterous aphid adults using water-based silver glue, aphids were tethered to the 2nd true leaf of a Kimmeridge plant, and an electrode was placed in the potting soil of the plant. This was placed in a Faraday cage to reduce electrical noise. The voltage fluctuations produced during aphid feeding were amplified using Direct Current Giga-8 systems (http://www.epgsystems.eu), recorded for eight hours with the EPG Stylet+ software (www.epgsystems.eu), the corresponding feeding behaviour was annotated according to their waveform patterns, and data were analysed with calculated using R with the adaptations that non-existing waveforms were assigned as zero values for total duration and as missing values for mean values. Behaviours that were interrupted by the end of a recording were included in the analyses. Each replicate was obtained by one aphid feeding on one plant. Preparing aphid treatments for EPG To obtain parasitised aphids (Bb.par and Mp.par) for the EPG experiment, 5-10 apterous aphids, a Kimmeridge leaf, and 2-5 mated parasitoid females were released inside a Petri dish, in line with previous experiments, we used D. rapae for B. brassicae aphids and A. colemani for M. persicae aphids .After observing a successful event of parasitism, the parasitised aphid was transferred to a Kimmeridge plant and kept there for 96 hours prior to the recordings. For the control group, aphid adults were picked from the same batch as parasitised aphids but directly placed on Kimmeridge plants until recording.

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2025-07-09
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