Raw MIBI and Nanostring GeoMx DSP images for human PDAC experiments

Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal and treatment-refractory cancer. Molecular stratification in pancreatic cancer remains rudimentary and does not yet inform clinical management or therapeutic development. In the attached manuscript, we construct a high-resolution molecular landscape of the multicellular subtypes and spatial communities that compose PDAC using single-nucleus RNA-seq and whole-transcriptome digital spatial profiling (DSP) of 43 primary PDAC tumor specimens that either received neoadjuvant therapy or were treatment-naive. Neoadjuvant therapy consisted of chemoradiotherapy with (CRTL) or without (CRT) losartan. We uncovered expression programs across malignant cells and fibroblasts, including a newly-identified neural-like progenitor malignant cell program that were enriched after chemotherapy and radiotherapy and associated with poor prognosis in independent cohorts. Integrating spatial and cellular profiles revealed three multicellular communities: classical, squamoid-basaloid, and treatment-enriched. Our refined molecular and cellular taxonomy can advance precision oncology in PDAC through stratification in clinical trials and as a roadmap for therapeutic targeting of specific cellular phenotypes and multicellular interactions.Here, we deposit the raw images from the multiplexed ion beam imaging (MIBI) and whole transcriptome (WTA) digital spatial profiling (DSP; Nanostring GeoMx) experiments. In the MIBI experiments (n=7), we analyzed 5 untreated and 2 treated samples. In the WTA DSP experiments (n=21), we analyzed 14 untreated and 7 treated samples.

胰腺导管腺癌（PDAC）是一种致命性极高且对治疗具有耐药性的癌症。胰腺癌的分子分层仍处于初级阶段，尚不能为临床管理或治疗开发提供信息。在所附的稿件中，我们利用单细胞RNA测序和全转录组数字空间分析（DSP）构建了由43例原发PDAC肿瘤样本组成的细胞亚型和空间群落的高分辨率分子景观，这些样本要么接受了新辅助治疗，要么未接受治疗。新辅助治疗包括含（CRTL）或不含（CRT）洛萨坦的化学放疗。我们发现，在恶性细胞和成纤维细胞中存在表达程序，包括一种新发现的类神经样祖细胞恶性细胞程序，这些程序在化疗和放疗后富集，并与独立队列中的不良预后相关。整合空间和细胞特征揭示了三个多细胞群落：经典型、鳞状-基底型和治疗富集型。我们精细的分子和细胞分类学可以促进胰腺癌的精准医学发展，通过临床试验中的分层以及作为针对特定细胞表型和多细胞相互作用的靶向治疗路线图。在此，我们存放了多路复用离子束成像（MIBI）和全转录组（WTA）数字空间分析（DSP；Nanostring GeoMx）实验的原始图像。在MIBI实验中（n=7），我们分析了5个未治疗和2个治疗样本。在WTA DSP实验中（n=21），我们分析了14个未治疗和7个治疗样本。