Data for Kabaria et al "Programmable Promoter Editing for Precise Control of Transgene Expression"

This deposit contains the raw data, analyzed data, and plasmid sequences for Kabaria et al "Programmable Promoter Editing for Precise Control of Transgene Expression." Additional information may be requested from the contact person. 'adam_attune.zip`,`Brittany_attune.zip`, `Emma.zip`, `Mary_1.zip`, `Mary_2.zip`, `Sneha_1.zip`,`Sneha_2.zip`, `Sneha_3.zip`, and `Yunbeen.zip` contain single cell flow cytometry data. Unzip and move to a new folder `attune_data` for use with the github repository described below. Some zips have been split into multiple for upload as indicated by underscore numbers. Combine these into the same folder in 'attune_data'. For example, all files in Mary_1 and Mary_2 should be put into a folder called Mary inside the 'attune_data' folder. `flow_summary_statistics FINAL.zip` contains the analyzed flow cytometry data for key experiments needed to generate the figures in the paper and perform statistics. These files or additional data can also be exported from the github below using the attune_data. Github code also contains the p-values from statistical tests. `Supplementary Data Sequences.xlsx` and `plasmid_gb.zip` contain plasmid sequences for this paper, as well as the sequences for variants of the DIAL and TET-DIAL promoters `unedited_gels_blots.zip` contains images of unedited blots and gels in the supplementary information of the research article. The code used to generate the figures from the data and modeling results can be found at https://github.com/GallowayLabMIT/Kabaria_Promoter_Editing_DIAL. The figure notebooks in this repository connect plate maps in .yaml files to folders with data in .csv files.