RNA-seq analysis for Chlamydomonas in ccm1 and cbp1 mutant

To identify genes affected by the CCM1 and CBP1 mutation, RNA-seq analysis was performed in C9 (WT), ccm1, cbp1 and their complemented strain. RNA was extracted from cells cultured under high-CO2 (HC) or Very low-CO2 (VLC) for 0.3 h or 2 h. There are two biological replicates of each condition. Sequencing was performed on Novaseq system and consisted of forward and reverse reads.