Staphylococcus aureus F98Y mutant dihydrofolate reductase complexed with NADPH and 2,4-diamino-5-[3-(3,4,5-trimethoxyphenyl)pent-1-ynyl]-6-methylpyrimidine (UCP115A)

Staphylococcus aureus F98Y mutant dihydrofolate reductase complexed with NADPH and 2,4-diamino-5-[3-(3,4,5-trimethoxyphenyl)pent-1-ynyl]-6-methylpyrimidine (UCP115A) Descriptor: 6-methyl-5-[3-methyl-3-(3,4,5-trimethoxyphenyl)but-1-yn-1-yl]pyrimidine-2,4-diamine, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, Trimethoprim-sensitive dihydrofolate reductase Authors: Anderson, A.C, Frey, K.M, Liu, J, Lombardo, M.N. Deposit date: 2009-01-07 Release date: 2009-03-24 Last modified: 2024-04-03 Method: X-RAY DIFFRACTION (1.77 Å) Cite: Crystal structures of wild-type and mutant methicillin-resistant Staphylococcus aureus dihydrofolate reductase reveal an alternate conformation of NADPH that may be linked to trimethoprim resistance. J.Mol.Biol., 387, 2009

金黄色葡萄球菌（Staphylococcus aureus）F98Y突变型二氢叶酸还原酶（dihydrofolate reductase）与烟酰胺腺嘌呤二核苷酸磷酸（NADPH）及2,4-二氨基-5-[3-(3,4,5-三甲氧基苯基)戊-1-炔基]-6-甲基嘧啶（UCP115A）形成的复合物。描述：6-甲基-5-[3-甲基-3-(3,4,5-三甲氧基苯基)丁-1-炔-1-基]嘧啶-2,4-二胺、烟酰胺腺嘌呤二核苷酸磷酸（NADPH）、甲氧苄啶（Trimethoprim）敏感型二氢叶酸还原酶。作者：Anderson, A.C.、Frey, K.M.、Liu, J.、Lombardo, M.N.。沉积日期：2009-01-07；发布日期：2009-03-24；最后修改日期：2024-04-03。实验方法：X射线衍射（分辨率1.77埃）。引用文献：《野生型与耐甲氧西林金黄色葡萄球菌（methicillin-resistant Staphylococcus aureus）突变型二氢叶酸还原酶的晶体结构揭示了可能与甲氧苄啶耐药相关的NADPH构象变体》，《J.Mol.Biol.（分子生物学杂志）》，第387卷，2009年。