GNPS - Daphnia dentifera - Metschnikowia bicuspidata host-pathogen system

The unexplained variation in susceptibility in several Daphnia species to multiple pathogens indicates that much remains to be learned about the Daphnia immune system. The goal of this experiment is to identify potentially important metabolites that contribute to the susceptibility or resistance of the host Daphnia dentifera to the fungal pathogen Metschnikowia bicuspidata. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 150 x 2.1 mm (Phenomenex, Torrance, CA). Data acquired in positive mode.